Journal: Nature

Article Title: Transcriptional Landscape of the Prenatal Human Brain

doi: 10.1038/nature13185

Figure Lengend Snippet: a . Nissl stained (right) and corresponding annotated reference atlas (left) plate, color coded by structure. b . ISH for RELN showing expression in Cajal-Retzius cells at low (left) and high (right) magnification in MZ. c . High resolution MRI and tract DWI of fixed ex cranio brain. d . Experimental strategy for systematic histology, anatomical delineation and LMD-based isolation of discrete anatomical structures for microarray analysis. Nissl, acetylcholinesterase (AChE) and GAP43 ISH were used to identify structures. e . Quantitative representation of microarray data for FOLR1, TGIF1 , and SIX3 . GE: ganglionic eminence. See for other anatomical abbreviations.

Article Snippet: Sample amplification, labeling, and microarray processing were performed by the Covance Genomics Laboratory (Seattle, WA).

Techniques: Staining, Expressing, Isolation, Microarray

Journal: Nature

Article Title: Transcriptional Landscape of the Prenatal Human Brain

doi: 10.1038/nature13185

Figure Lengend Snippet: a. Fluorescent in situ hybridization (FISH) in proliferative layers of 15 post-conceptual week human cortex for three genes in modules G7 and G8 in the germinal layers network shown in of the manuscript (see )—SPATA13, NR2E1, and DTL. All three genes show enrichment in the VZ compared to the SZ as expected based on microarray data. Nuclei are labeled with DAPI (blue). b. High magnification images in the VZ show double labeling for each pair of genes (with fluor reversal, lower row) and show complex subcellular distributions. SPATA13, NR2E1, and (to a lesser extent) DTL appear to be expressed in most cells in the VZ, but these genes are typically expressed in non-overlapping punctate cytoplasmic locations (excluded from DAPI-stained nuclei in blue). b is at 50× magnification relative to a .

Article Snippet: Sample amplification, labeling, and microarray processing were performed by the Covance Genomics Laboratory (Seattle, WA).

Techniques: In Situ Hybridization, Microarray, Labeling, Staining

Journal: Nature

Article Title: Transcriptional Landscape of the Prenatal Human Brain

doi: 10.1038/nature13185

Figure Lengend Snippet: a . NPY is enriched in SP at 21pcw but not 15–16pcw based on microarray (left) and ISH (right). Microarray data is plotted as the average +/− standard error of the mean (SEM) for each layer in each brain. b . Genes with SP enrichment in both species, based on microarray data in human (upper row) and ISH data at E15.5 or E18.5 mouse (lower row). c . Genes with SP enrichment in human but either no expression ( DKK1, CRTR2 and MESP1 ) or no SP enrichment ( CHD1 and CRYM ) in mouse. d . Genes with SP enrichment in developing mouse, but not human, SP. Asterisks indicate common expression between human and mouse in other layers (i.e., SG/pia mater). Mouse ISH images taken from the Allen Developing Mouse Brain Atlas.

Article Snippet: Sample amplification, labeling, and microarray processing were performed by the Covance Genomics Laboratory (Seattle, WA).

Techniques: Microarray, Expressing

Journal: Nature

Article Title: Transcriptional Landscape of the Prenatal Human Brain

doi: 10.1038/nature13185

Figure Lengend Snippet: ( a ) Novel human subplate-enriched genes showing at least 8-fold enrichment in SP in all four prenatal human brains. CDH18 , a known SP marker in mouse, is presented as a positive control. ( b ) Genes with differences in subplate expression between mouse and human. These genes have been reported as subplate-enriched in mouse studies but do not show human subplate enrichment. Labeling as in of the main manuscript. Microarray data is plotted as the average +/− standard error of the mean (SEM) for each layer in each of the four brains analyzed (colors).

Article Snippet: Sample amplification, labeling, and microarray processing were performed by the Covance Genomics Laboratory (Seattle, WA).

Techniques: Marker, Positive Control, Expressing, Labeling, Microarray