Journal: Scientific Reports

Article Title: Pre-exposure of Arabidopsis to the abiotic or biotic environmental stimuli “chilling” or “insect eggs” exhibits different transcriptomic responses to herbivory

doi: 10.1038/srep28544

Figure Lengend Snippet: ( a ) Principle component analysis of log 2 -transformed microarray data of individual samples (biological replicates) from non-treated, chilling- or egg-treated leaves after three days recovery (C 3 , P 3 *, P 3 E) and the respective samples that were exposed to Pieris brassicae larval feeding (T, P 3 * + T, P 3 E + T). Depicted are the first two principal components PC1 and PC2 which explain 29.5% and 16.6% of the variance, respectively. Ellipses represent 95% confidence intervals. ( b ) Genes regulated by larval feeding on untreated plants (T/C 3 ), by larval feeding on prior egg-treated plants (P 3 E + T/C 3 ) and differentially regulated genes by larval feeding on prior egg-treated plants compared to larval feeding on untreated plants (P 3 E + T/T). ( c ) Genes regulated by larval feeding on untreated plants (T/C 3 ), by larval feeding on prior chilling-treated plants (P 3 * + T/C 3 ) and differentially regulated genes by larval feeding on prior chilling-treated plants compared to larval feeding on untreated plants (P 3 *T/T). Depicted are genes with expression ratios ≥2 and P adj < 0.05 ( n = 3; except for C 3 with n = 4).

Article Snippet: Labelling of total RNA and microarray processing were performed by Oaklabs GmbH.

Techniques: Transformation Assay, Microarray, Expressing

Journal: International Journal of Biological Sciences

Article Title: Upregulation of miR-335-3p by NF-κB Transcriptional Regulation Contributes to the Induction of Pulmonary Arterial Hypertension via APJ during Hypoxia

doi: 10.7150/ijbs.34517

Figure Lengend Snippet: Chronic normobaric hypoxia (CNH) exposure induces pulmonary arterial hypertension (PAH) and upregulates miR-335-3p expression in the lungs of mice. CNH treatment significantly increased RVSP (A) and RV/(LV+S) (B) (N=5-8 per group). (C) Heatmap and sample clustering analysis of the differential expressed miRs in the lungs of mice (N=4 per group). Each row represents a miR and each column represents a sample. (D) Microarray and (E) quantitative RT-PCR analysis of miR-335-3p expression in the lungs of mice. (F) Correlation analysis between miR-335-3p and RVSP, and (G) Correlation analysis between miR-335-3p and RV/(LV+S) (N=4 per group). Values shown are means±s.e.m. ** p <0.01, *** p <0.001 vs. Normoxia.

Article Snippet: Microarray processing and analysis for miRs was performed by the Ribobio Co., Ltd. (Guangzhou, China).

Techniques: Expressing, Microarray, Quantitative RT-PCR